Required Research Scientist - Enzyme Immobilization, Biocatalytic Route Design & Enzyme Engineering (02 Vacancies)-M.Pharm/M.Sc/M.Tech (Biotechnology/Biochemistry/Microbiology)

Bachwell Pharma is hiring Research Scientist - Enzyme Immobilization, Biocatalytic Route Design & Enzyme Engineering (02 Vacancies)

Details are provided below

1.Position: Research Scientist -

Enzyme Immobilization, Biocatalytic Route Design & Enzyme Engineering (02 Vacancies)

2. Department: Hands-on Enzyme Immobilization Execution, Stability, Mechanics & Kinetic Evaluation, Route Design Verification & High-Throughput Screening, Documentation, Maintenance & Lab Support

3. Experience: 1 - 4 years

4. Location: Genome Valley Hyderabad, India

5. Qualifications:

M.Pharm/M.Sc/M.Tech (Biotechnology/Biochemistry/Microbiology)

6. Core Job Responsibilities

A. Hands-on Enzyme Immobilization Execution

Carrier Matrix Preparation & Screening: Prepare and condition various solid supports (such as hydrophobic macroporous resins, acrylic beads, silica matrices, and magnetic nanoparticles) for immobilization runs.

Execution of Binding Protocols: Conduct diverse immobilization techniques including physical adsorption, ionic binding, entrapment (e.g., alginate beads), and covalent attachment using cross-linking agents like glutaraldehyde.

CLEA Formulation: Support the formulation and preparation of Cross-Linked Enzyme Aggregates (CLEAs) and carrier-free immobilized preparations to maximize enzyme loading.

Mass Balance Calculation: Calculate and optimize immobilization parameters, specifically evaluating Immobilization Yield (the percentage of enzyme bound to the support) and Expressed Activity (how active the enzyme remains after being bound).

B. Stability, Mechanics & Kinetic Evaluation

Recyclability Trials: Run multi-cycle batch reactions to determine the

4G+ 40 structural and mechanical reusability of the immobilized biocatalyst, tracking operational half-life and activity decay over successive rounds.

Leaching Analysis: Monitor and analyze reaction supernatants using protein assays (e.g., Bradford or BCA assays) to ensure the enzyme is tightly bound and not leaching off the support matrix into the product stream.

Altered Kinetics Testing: Run time-course sampling to generate raw data for apparent kinetics modeling (Kapp_m, Vapp_max), specifically testing for mass transfer limitations and diffusion constraints under different stirring speeds or flow rates.

C. Route Design Verification & High-Throughput Screening Biotransformation Assays: Set up and monitor parallel, microscale enzymatic reactions (using automated liquid handlers or deep-well plates) to screen immobilized enzyme libraries against target chemical intermediates.

Reaction Media Setup:

Analytical In-Process Controls (IPC):

D. Documentation, Maintenance & Lab Support

Meticulous Data Logging:

Equipment Care: Operate and maintain key instrumentation required for immobilization workflows, such as freeze-dryers (lyophilizers), particle size analyzers, automated titrators, and cross-flow filtration assemblies.

EHS Adherence:

7. How to Apply:

Interested candidates can share their resume at sreddy@bachwellpharma.com (main mail ID), hr@bachwellpharma.com, bachwellpharma@gmail.com

Click Here for Details

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